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Which characteristic determines spatial resolution limits for stimulated emission depletion microscopy?

A)Point spread function contraction
B)Fluorophore blinking rate reduction
C)Photo bleaching reaction threshold
D)Excitation-emission time-delay broadening

💡 Explanation

Resolution improves because stimulated emission narrows the point spread function; therefore, diffraction limits contraction rather than photobleaching or emission delays. The resolution increases due to PSF narrowing, not other factors.

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