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← ScienceWhich characteristic determines spatial resolution limits for stimulated emission depletion microscopy?
A)Point spread function contraction✓
B)Fluorophore blinking rate reduction
C)Photo bleaching reaction threshold
D)Excitation-emission time-delay broadening
💡 Explanation
Resolution improves because stimulated emission narrows the point spread function; therefore, diffraction limits contraction rather than photobleaching or emission delays. The resolution increases due to PSF narrowing, not other factors.
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