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← ScienceWhich consequence results when intramolecular hydrogen bonding dominates enzyme active sites?
A)Reduced substrate binding affinity✓
B)Increased catalytic turnover rate
C)Enhanced protein thermal stability
D)Greater solvent accessibility achieved
💡 Explanation
Reduced substrate affinity occurs because pre-existing intramolecular hydrogen bonds within enzyme active sites hinders substrate binding rather than promoting it. Therefore, a substrate has to overcome greater energetic barriers rather than easily fitting, decreasing binding; substrate affinity worsens.
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