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← ScienceWhich consequence would result when manipulating enzyme active site proximity?
A)Lowered temperature denatures its quaternary structure
B)The Michaelis constant Km is affected✓
C)The equilibrium favors product formation overall
D)Increased cofactor affinity decreases total activity
💡 Explanation
Changing the active site proximity affects substrate binding because the enzyme-substrate complex formation alters as a function of active site efficiency, and therefore changes the Michaelis constant Km, rather than affecting denaturation or equilibrium directly.
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