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Which mechanism inhibits methane production when anaerobic digester temperature fluctuates excessively?

A)Enzyme denaturation reduces hydrolysis rate
B)Substrate depletion limits methanogenesis
C)Product inhibition slows acetate uptake
D)pH shift disrupts cell membrane transport

💡 Explanation

When temperature fluctuates, enzyme denaturation occurs because bonds maintaining protein structure are broken, reducing activity in hydrolysis and acidogenesis stages leading to inhibited methanogenesis. Therefore, a reduced hydrolysis rate results, rather than others which would need specific substrate/product imbalances or disruptions to pH balance.

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