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Which mechanism limits spatial resolution in transmission electron microscope despite short wavelength?

A)Chromatic aberration in objective lens
B)Specimen charging causing beam deflection
C)Electron orbital diffraction by atoms
D)Thermal noise affecting detector sensitivity

💡 Explanation

When electrons pass through the objective lens, chromatic aberration occurs because variations energy affect the focal length giving blurring, limiting the resolution despite De Broglie wavelength reduction. Therefore chromatic aberration limits resolution, rather than orbital diffraction, charging or thermal noise which require different failure context.

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