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Which mechanism slows down enzyme activity upon cooling in fish?

A)Reduced conformational flexibility disables catalysis
B)Increased solvent volume enables denaturation
C)Metabolic regulation enforces substrate binding
D)Active transport floods catalysis centres

💡 Explanation

Decreasing temperature reduces the thermal energy available for enzymes; therefore, *thermal fluctuation* lowers the chance that enzymes achieve proper conformation for the active site to catalyse reactions, because stability, rather than the solvation of the protein is the determining factor.

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