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Which outcome limits an enzyme's catalytic rate inside cells?

A)Substrate diffusion to the enzyme
B)Product molecule conformational instability
C)Enzyme denaturation by local acidification
D)Inhibitor binding at allosteric sites

💡 Explanation

The reaction reaches maximal velocity (Vmax) when substrate arrival becomes rate-limiting, known as diffusion limitation, because the enzyme active site is unoccupied a significant amount of time; therefore substrate diffusion restricts catalysis, rather than conformational instability playing the dominant role.

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