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Which outcome limits biofuel production from algae using lipase?

A)Enzyme active site denaturation
B)Substrate diffusion rate decreasing
C)Product equilibrium shifts unfavorably
D)Viscosity increase inhibits mixing

💡 Explanation

Reaction rate decreases because enzyme active sites are disrupted by excessive heat via thermal unfolding, a mechanism of protein denaturation; therefore the conversion rate slows, rather than equilibrium shifts, caused by changes in concentration.

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