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Which outcome limits resolution during biological AFM measurements?

A)Tip-sample contact area broadens
B)Cantilever stiffness greatly increases
C)Thermal noise severely damages sample
D)Laser spot significantly defocuses

💡 Explanation

Increased contact area causes lower resolution because repulsive electron cloud interference broadens the sample interaction area, masking fine topological details; therefore resolution decreases rather than from laser effects or cantilever properties under operating constraints.

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