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← ScienceWhich outcome occurs to an enzyme at high ionic strength?
A)Altered protein folding and aggregation✓
B)Increased substrate reaction catalysis
C)Enhanced protein solubility is observed
D)Reduced active site binding stability
💡 Explanation
High ionic strength disrupts electrostatic interactions, causing salting out via the Debye-Hückel screening, because of the disrupted function of its typical charge distribution; therefore, the protein aggregates rather than maintaining stable folding, because the protein unfolds due to disruptions.
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