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Which outcome occurs when an irreversible enzymatic reaction reaches a substrate saturation limit?

A)Reaction rate approaches a maximum constant
B)Enzyme-substrate affinity decreases gradually
C)Product inhibition decreases dynamically
D)Michaelis constant decreases significantly

💡 Explanation

The reaction rate will plateau because enzyme active sites become saturated; Michaelis-Menten kinetics predict that reaction rate approaches a maximum constant concentration. This rate is dependent on enzyme concentration, rather than substrate availability, therefore rate approaches maximum rather than any change to constants.

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