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← ScienceWhich outcome occurs when pressure increases during high-performance liquid chromatography?
A)Solute precipitation obscures the detector
B)Molecular interactions intensify; separation improves✓
C)Viscosity decrease aids analyte elution
D)Mobile phase changes impacting selectivity
💡 Explanation
Increased pressure compresses the mobile phase, which intensifies molecular interactions due to reduced intermolecular distances—increasing the efficiency of analyte partitioning. Therefore, resolution improves at higher pressures, rather than a decrease in separation efficiency at altered selectivity or viscosity.
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