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← ScienceWhich outcome occurs when reversible enzyme-substrate binding reaches saturation?
A)Vmax achievement, reaction equilibrium constant✓
B)Enzyme denaturation, irreversible conformation change
C)Active site blocking, decreased substrate affinity
D)Product inhibition, complete catalytic cycle
💡 Explanation
Vmax is reached because, with saturation, all enzyme active sites are occupied; the rate is limited by product formation, causing an equalization with reactants through Le Chatelier's principle. The reaction reaches equilibrium constant rather than the enzyme denaturing or reduced affinity, under normal enzyme conditions.
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