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← ScienceWhich outcome reduces data readout accuracy when using a Coherent anti-Stokes Raman Scattering (CARS) microscope?
A)Non-resonant background intensity interfering strongly✓
B)Increased photobleaching of the sample volume
C)Lower signal strength at high frequencies
D)Thermal lensing causing image distortion
💡 Explanation
A strong non-resonant background signal occurs because of electronic hyperpolarizability, leading to constructive interference of the signals, rather than destructive interference. Therefore, the CARS signal is contaminated, rather than providing a high signal-to-noise readout.
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