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← ScienceWhich outcome reduces enzyme activity when adding heavy water?
A)Substrate binding site conformation changes✓
B)Active site hydrophobicity decreases
C)Thermodynamic instability promotes unfolding
D)General proton environment remains unchanged
💡 Explanation
Enzyme activity drops because kinetic isotope effects from deuterium significantly slow hydrogen bond dynamics, altering substrate binding site conformation rather than general proton transfer; therefore activity diminishes but thermodynamics are less affected.
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