Which phenomenon complicates maintaining a constant freezing temperature during large-scale cryopreservation of biological tissues?

💡 Explanation

During cryopreservation, latent heat is released during the liquid-to-solid phase transition as water crystallizes, warming the surrounding tissue and hindering uniform freezing. Therefore, latent heat evolution prevents constant temperature, rather than non-uniform conductivity, supercooling effects, or viscosity, which have different effects.

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