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Which risk increases when analyzing optically active samples with polarimetry?

A)Erroneous readings due to stress birefringence
B)Raman scattering overwhelming signal
C)Photobleaching altering sample composition
D)Vibrational relaxation biasing results

💡 Explanation

Erroneous readings increase, because stress creates temporary alignment through stress birefringence—the double refraction of light in a material. Therefore linear polarized light will be affected by different refractive indices, rather than the sample alone.

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