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← ScienceWhich risk increases when analyzing optically active samples with polarimetry?
A)Erroneous readings due to stress birefringence✓
B)Raman scattering overwhelming signal
C)Photobleaching altering sample composition
D)Vibrational relaxation biasing results
💡 Explanation
Erroneous readings increase, because stress creates temporary alignment through stress birefringence—the double refraction of light in a material. Therefore linear polarized light will be affected by different refractive indices, rather than the sample alone.
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