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Which risk increases when back-pressure causes a rate limitation in recombinant protein fermentation?

A)Nutrient gradients become acutely inhibitory
B)Cell lysis from mechanical shear decreases
C)Substrate diffusion is uniformly enhanced
D)Temperature regulation will exhibit homeostasis

💡 Explanation

Harmful nutrient gradients build as back-pressure bottlenecks waste removal, suppressing cell performance via 'Rate-Limiting Enzyme Inhibition'. Therefore gradients elevate rather than diffusion assisting, because cell death relies on alternative mechanical disruption processes.

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