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← ScienceWhich risk increases when using rapid steam pressure variation for sterilization?
A)Cellular lysis by osmotic shock✓
B)Protein denaturation incomplete unfolding
C)Increased enzyme catalyzed reactions
D)Reversed RNA transcription processes enhanced
💡 Explanation
Rapid pressure shifts in steam sterilization induce osmotic shock, because of the microorganism's cell membrane instability during liquid to vapor phase transition. Therefore, cellular lysis increases, rather than simply causing protein denaturation, which requires higher temperatures, or enhanced enzyme reactions – as those require specific catalyst.
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