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Which consequence results when mismatch occurs between fluorophore emission/sensor absorption functions?

A)Reduced FRET efficiency observed
B)Increased signal-to-noise ratio occurs
C)Faster fluorophore photobleaching transpires
D)Ratiometric measurement errors amplified

💡 Explanation

Reduced FRET efficiency happens because dipole-dipole interactions require spectral overlap, impairing Förster resonance energy transfer; therefore, the energy transfer suffers, rather than enhanced signal or photobleaching, due to inefficiency.

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