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← ScienceWhich phenomenon limits electron microscope resolution when imaging biological macromolecules at cryogenic temperatures?
A)Diffraction from finite objective aperture
B)Inelastic scattering from specimen✓
C)Chromatic aberration from energy spread
D)Brownian motion of hydration shell
💡 Explanation
When electrons interact with matter, inelastic scattering occurs because electrons lose energy through interactions that decreases coherence, adding to blurring and limiting obtainable resolution. Therefore inelastic scattering limits resolution, rather than other effects which are mitigated with better lenses or specimen preparation techniques.
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