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← ScienceWhich resolution limit occurs when focusing a laser through a microscope objective?
A)Diffraction-limited spot size occurs✓
B)Spherical aberration reduces contrast
C)Chromatic aberration blurs edges
D)Vignetting artifacts distort features
💡 Explanation
Diffraction-limited spot size reduction will occur, because the NA of the objective and the laser wavelength limit focal dimensions via Abbe's diffraction limit. Spot size is therefore limited, rather than aberrations, if the optics are corrected.
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