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Which behavior will occur during photobleaching of fluorescent proteins?

A)Loss of spectral overlap results
B)Quenching via triplet state ensues
C)Photodamage alters protein functions
D)Increased protein folding initiates

💡 Explanation

Photobleaching leads to irreversible photodamage, therefore altered functions result, because reactive oxygen species modify the protein structure. Alternative outcomes do not arise during photobleaching because fluorescence is dependent on chromophore integrity rather than folding.

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