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Which outcome limits optical resolution in molecular imaging?

A)Diffraction from finite aperture size
B)Aberrations in the excitation beam
C)Quantum yield saturation threshold
D)Photobleaching of fluorophore molecules

💡 Explanation

Diffraction limits resolution because light waves spread as the light passes through the objective aperture, creating a blurred image, rather than a perfect point. Therefore, diffraction determines the achievable resolution limit, because other effects such as aberrations contribute to image degradation.

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