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← ScienceWithin high-performance liquid chromatography (HPLC), which outcome results when target molecules lack sufficient mobile phase interaction?
A)Peak broadening reduces signal resolution✓
B)Elution order alters unpredictably
C)Column pressure increases exponentially
D)Mobile phase viscosity dramatically rises
💡 Explanation
Peak broadening reduces signal resolution because insufficient mobile phase interaction impedes proper separation, leading to molecules eluting closely together due to inadequate partitioning via intermolecular forces along stationary phase; therefore peaks broaden, rather than maintain narrow widths with correct resolution that occurs upon correct partitioning.
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